Two-strokes like the Junkers and Deltic need a scavenge blower or supercharger. But there are many two-strokes designs that don't.I'm surprised no-one has mentioned the Napier Deltic; three banks of six cylinders (each with two pistons) arranged in a triangle, giving 1750Hp. The triangular arrangement meant each bank of cylinders had a crankshaft at each end but saved weight as there were only three crankshafts for three banks. It did need a supercharger (and compressed air starting) to work, and gave good service in the Dark class FPBs and, of course, the Deltic class Locomotives.
SRJ.
It needed a scavenging pump, as do all two-stroke engines. Compressed air starting isn't that uncommon on large or very large diesels.
Early engines used vertical loop scavenging. The inlet and exhaust ports were separated by a piston with a steep, wedge-like crown that was meant to deflect the incoming charge up and the exhaust down on the opposite side. It did not work very well and produced problems with combustion-chamber shape and piston heating.
Modern, horizontal loop- and cross-port-scavenging designs place and size ports to take advantage of the gas flow in the engine. Tuned inlet and exhaust pipes are also used to aid scavenging through resonance.
A supercharger or turbocharger probably makes for a cleaner and more fuel-efficient engine. But it is not essential.
Any text book on engine design can provide this information.